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Related Experiment Video

Updated: May 8, 2026

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair
09:34

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair

Published on: September 7, 2017

High-Performance Prevascularized SHED-Laden rGO@Hydrogel Achieves Optimized Diabetic Bone Defect Repair.

Can Zhang1, Yiyuan Kang1,2, Shulin Lai1

  • 1Stomatological Hospital, Southern Medical University, Guangzhou, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|May 7, 2026
PubMed
Summary

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This study developed a novel scaffold using reduced graphene oxide (rGO) to enhance blood vessel formation and bone repair in diabetic patients. The rGO-integrated hydrogel significantly improved healing in a diabetic dog model, offering a new strategy for bone regeneration.

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Diabetic bone defects exhibit impaired angiogenesis and osteogenesis, hindering repair.
  • Existing tissue-engineered scaffolds struggle with vascularization in hyperglycemic conditions.
  • Endothelial cells' angiogenic capacity is compromised in diabetic microenvironments.

Purpose of the Study:

  • To develop a prevascularized scaffold for enhanced bone repair in diabetic conditions.
  • To investigate the role of reduced graphene oxide (rGO) in scaffold-mediated vascularization and osteogenesis.
  • To validate the therapeutic efficacy in a diabetic animal model.

Main Methods:

  • Encapsulation of stem cells from human exfoliated deciduous teeth (SHED) within an rGO-integrated hydrogel.
Keywords:
bone regenerationdiabetesextracellular matrixprevascularized scaffoldreduced graphene oxidestem cells from the human exfoliated deciduous teeth

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Last Updated: May 8, 2026

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair
09:34

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair

Published on: September 7, 2017

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Half-segmental Diaphyseal Bone Defect Model in Rats for Evaluating Bone Substitute Performance in Load-bearing Regions

Published on: December 30, 2025

  • In vitro assessment of vascular network formation.
  • Evaluation in a diabetic beagle dog mandibular defect model.
  • Mechanistic studies involving the FAK-Src/RELA pathway and P4HA1 upregulation.

    • Main Results:

    • rGO significantly accelerated SHED-mediated vascular network formation in vitro.
    • The rGO-integrated scaffold demonstrated increased vascular density and accelerated bone regeneration in vivo.
    • Mechanistic studies revealed rGO activates the FAK-Src/RELA pathway, upregulating P4HA1 and enhancing collagen I synthesis.
    • rGO promotes extracellular matrix remodeling, creating a pro-angiogenic niche.

    Conclusions:

    • Engineering the extracellular matrix with rGO is a novel strategy for accelerating prevascularization and bone repair in diabetic conditions.
    • The developed scaffold shows promise for treating diabetic bone defects.
    • This approach enhances the angiogenic capacity within a pro-regenerative scaffold.