Comparative Oligo-FISH Mapping Illuminates Chromosomal Evolution Among Rutaceae Species Diverged Over 50 Million

Li He1, Hainan Zhao2, Xiaoxue Zeng1

  • 1Horticulture Research Institute, National-local Joint Engineering Laboratory of Citrus Breeding, Cultivation, Key-Laboratory of Horticultural Crops Biology and Germplasm Enhancement in Southwest China, Ministry of Agriculture and Rural Affairs, Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops of Sichuan Province, Sichuan Academy of Agricultural Sciences, Chengdu, China.

Summary

Oligo-based fluorescence in situ hybridization (FISH) probes developed in citrus successfully mapped chromosomes in related species, revealing evolutionary histories. This method enables rapid reconstruction of plant chromosomal evolution across deep divergence times.

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