Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 8, 2026

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
05:57

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

Published on: May 16, 2011

Measuring C. difficile Adhesion to Mucosal Surfaces.

Ben S Sidner1,2, Hugh C McCullough1,2, Baishakhi Biswas1,2

  • 1Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 7, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cadmium toxicity to the human gut microbiome varies depending on composition.

Applied and environmental microbiology·2026
Same author

Insight into the relationship between type IV pilus function and biofilm formation.

Biochemical Society transactions·2026
Same author

Predicting Interspecies Metabolic Dependencies in Microbial Communities by Integrating Flux Coupling Analysis with SteadyCom.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

LambdaPy and LambdaR: Thermodynamics-Based Biogeochemical Reaction Modeling Packages for Integrating High-Resolution Mass Spectrometry Data.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

An In Vitro Model for Studying Interactions Between Gastrointestinal Microbes and Planktonic and Sessile Clostridioides difficile Populations.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

<i>Limousia</i> bacteria encode mucinolysome for mucin utilization in animal gut microbiomes.

Gut microbes·2026
This summary is machine-generated.

Clostridioides difficile attachment to host tissues is key for colonization. This study details methods to measure its binding to mucin hydrogels in vitro.

Area of Science:

  • Microbiology
  • Gastroenterology
  • Biomaterials Science

Background:

  • Clostridioides difficile colonization requires attachment to host tissues.
  • Attachment mechanisms may involve multiple modes, host cells, and bacterial aggregation.
  • Understanding C. difficile attachment is crucial for persistence during infection or asymptomatic colonization.

Purpose of the Study:

  • To describe methods for directly measuring in vitro attachment of Clostridioides difficile.
  • To investigate C. difficile attachment to mucin hydrogels from various sources.

Main Methods:

  • Development and description of in vitro methods.
  • Utilizing mucin hydrogels as a model for host mucosa.
  • Direct measurement of Clostridioides difficile attachment to these hydrogels.
Keywords:
AdhesionGlycobiologyHost-pathogen interactionsMucin

More Related Videos

Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease
08:27

Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease

Published on: September 20, 2018

Related Experiment Videos

Last Updated: May 8, 2026

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
05:57

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

Published on: May 16, 2011

Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease
08:27

Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease

Published on: September 20, 2018

Main Results:

  • Established methods for quantifying C. difficile attachment to mucin hydrogels.
  • Demonstrated the feasibility of studying C. difficile-mucin interactions in vitro.
  • Provided a foundation for further research into attachment mechanisms.

Conclusions:

  • Direct in vitro measurement of Clostridioides difficile attachment to mucin is achievable.
  • This methodology facilitates the study of host-pathogen interactions.
  • Further research can elucidate specific attachment factors and strategies.