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Related Experiment Video

Updated: May 9, 2026

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods
10:46

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods

Published on: May 2, 2016

Quad-Channel In Vivo Photoacoustic Multiplexing Using Tunable Gold Nanorods.

Anamik Jhunjhunwala1, Myeongsoo Kim1,2,3, Huyju Mun1

  • 1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, Atlanta, Georgia 30332, United States.

ACS Nano
|May 7, 2026
PubMed
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This summary is machine-generated.

This study introduces novel gold nanorods for enhanced photoacoustic (PA) imaging, enabling four-channel multiplexing. These advanced contrast agents improve spectral separation and accuracy for in vivo molecular imaging applications.

Area of Science:

  • Biomedical Imaging
  • Nanotechnology
  • Optical Spectroscopy

Background:

  • Photoacoustic (PA) imaging offers molecular specificity and ultrasound resolution for noninvasive in vivo imaging.
  • Current PA multiplexing is limited by contrast agent spectral properties and endogenous absorber interference.

Purpose of the Study:

  • To develop a robust four-channel PA multiplexing platform using spectrally distinct gold nanorods.
  • To overcome limitations of existing contrast agents and endogenous absorbers for improved PA imaging.

Main Methods:

  • Synthesized PEGylated silica-coated gold nanorods with narrow, tunable plasmon resonances in the near-infrared window.
  • Employed a seed-mediated synthesis and silica encapsulation for enhanced photostability and standardized particles.
Keywords:
contrast agentsgold nanorodsin vivo molecular imagingmultiplexingphotoacoustic imagingsilica coatingspectral unmixing

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  • Utilized a non-negative least-squares algorithm for accurate signal separation in vitro and in vivo.

    • Main Results:

    • Achieved four-channel PA multiplexing with mean absolute errors below 4.3%.
    • Demonstrated spectral distinguishability in the presence of endogenous hemoglobin.
    • Successfully unmixed signals using commercial spectral deconvolution methods.

    Conclusions:

    • Developed a versatile platform doubling PA multiplexing capabilities to four exogenous channels.
    • Established a framework for advanced noninvasive biomarker mapping and diagnostics.
    • Showcased the potential for simplified, high-performance PA imaging without complex setups.