Related Experiment Video For Carbon nanotube
Updated: May 9, 2026
Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
Published on: October 10, 2013
Carbon nanotube field-effect transistor-based immunosensor for highly sensitive detection of interleukin-6 in
Syed Kashif Ali1, Gulam Rabbani2, Akbar Mohammad2
1Department of Physical Sciences, Chemistry Division, College of Science, Jazan University, P.O. Box. 114, Jazan 45142, Saudi Arabia; Nanotechnology Research Unit, Jazan University, P.O. Box. 114, Jazan 45142, Saudi Arabia.
Abstract:
Interleukin-6 (IL-6) is an inflammatory cytokine that plays a crucial role in cancer, with its elevated level being indicative of metastasis. Oral squamous cell carcinoma (OSCC) is a major type of head and neck cancer and IL-6 has been identified as a critical factor that is overexpressed in its development, progression and metastasis. IL-6 is also associated with various pathological processes, such as chronic inflammation, cancer and severe COVID-19 infection. This study aims to develop an affordable, simple manufacturing process for a user-friendly, label-free electrochemical immunosensor to detect IL-6 in a real-time manner in artificial saliva. Carbon nanotube field-effect transistor (CNT-FET)-based sensor was fabricated and transfer and output curves were measured and the device sensor exhibited p-type semiconductor properties. Under optimized experimental parameters, the fabricated CNT-FET-based immunosensing platform can detect IL-6 antigen with a wide detection range from 1.0 to 300 pg/mL with a relatively low detection limit (LOD) of 1.15 pg/mL in 1.0 mM PBS. Additionally, the developed immunosensor showed outstanding specificity, sensitivity, good repeatability and high stability in 1.0 mM PBS. Furthermore, the fabricated immunosensor was successfully used in artificial saliva samples spiked with IL-6 and recovery percentages were in the range of 102 to 104.5%.
