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Updated: May 9, 2026
A Mouse Ear Model for Allergic Contact Dermatitis Evaluation
Published on: March 24, 2023
Efficacy of Xiao-Feng Powder-loaded thermosensitive composite hydrogel alleviates allergic contact dermatitis by
Yingxin Long1, Wenzhang Dai1, Hui Ding1
1State Key Laboratory of Bioactive Molecules and Druggability Assessment, Guangdong Basic Research Center of Excellence for Natural Bioactive Molecules and Discovery of Innovative Drugs/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China, College of Pharmacy, Jinan University, Guangzhou, Guangdong, 510632, China.
Background:
Allergic contact dermatitis (ACD) is an immunological disorder elicited by antigen exposure, which activates the immune system and leads to T cell-mediated inflammation.
Purpose:
This study aimed to investigate the role of Xiao-Feng Powder (XFP)-loaded thermosensitive composite hydrogel in alleviating ACD and elucidated its underlying mechanisms in HaCaT cell induced by TNFα/IFNγ.
Methods:
SADBE-induced ACD mouse and TNFα/IFNγ-induced damage to HaCaT cells were established. CFB expression was knocked down in HaCaT cells via shRNA plasmid transfection. UPLC-MS was employed to analyze the chemical composition of the TCM compound XFP. The composite F127-PEG200/XFP@SA-GEL-PVP was prepared by encapsulating XFP in microspheres via a blend of sodium alginate (SA), gelatin (GEL), and polyvinylpyrrolidone (PVP), followed by their integration into a hydrogel matrix of Pluronic F127 (F127) and PEG200. Scratching behavior, skin fold thickness, spleen-thymus index, histological analysis, photoacoustic imaging system were used to evaluate the effect of F127-PEG200/XFP@SA-GEL-PVP. Finally, expression levels of CFB, RANKL, IL4, IL13, TSLP, and IL25 were analyzed by immunohistochemistry and western blot.
Results:
CFB regulates Th2-type cytokine expression via C3 in the TNFα/IFNγ-induced HaCaT cells. F127-PEG200/XFP@SA-GEL-PVP exhibited stable physicochemical properties and favorable biocompatibility. Treatment with F127-PEG200/XFP@SA-GEL-PVP significantly reduced scratching behavior, skin fold thickening, and inflammatory infiltration in ACD mice, inhibited mast cell degranulation, restored vascular morphology and local blood flow in skin and spleen tissues, and downregulated the expression levels of Th2-type cytokines (IL4, IL13, TSLP) and pro-inflammatory mediators (CFB, RANKL, IL25).
Conclusion:
These findings demonstrate that F127-PEG200/XFP@SA-GEL-PVP exerts protective effects in ACD. Silencing CFB restored HaCaT cell viability after TNFα/IFNγ treatment and the modulation of the C3 pathway reduced Th2-type cytokine expression.
