AtGATA11 activates AtBCCP2 transcription in Arabidopsis in response to NaHCO₃ stress

NaHCO₃-induced alkaline stress severely affects plant growth by imposing ionic, osmotic, and high-pH stress. However, the transcriptional mechanisms underlying plant responses to NaHCO₃ remain incompletely understood. In this study, RNA sequencing was performed to analyze transcriptomic changes in shoots and roots of wild-type Arabidopsis thaliana treated with 3 mM NaHCO₃ for 0, 4, 12, 24, and 48 h. Among the differentially expressed genes, AtBCCP2, encoding biotin carboxyl carrier protein 2, was identified as a NaHCO₃-responsive gene. Co-expression network analysis predicted several transcription factors potentially regulating AtBCCP2. Yeast one-hybrid and dual-luciferase assays demonstrated that the transcription factor AtGATA11 directly binds to the promoter of AtBCCP2 and activates its transcription. Notably, AtGATA11-mediated transcriptional activation of AtBCCP2 was significantly enhanced under NaHCO₃ treatment. These results identify AtGATA11 as a direct transcriptional regulator of AtBCCP2 during NaHCO₃ stress and provide molecular evidence for transcription factor mediated regulation in plant alkaline stress responses.