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Updated: May 9, 2026

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

Atomic force microscopy imaging of RBL-2H3 cell degranulation.

Jiani Li¹, Jing Hu¹, Yujuan Chen¹

¹International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun, 130022, China; Centre for Opto/Bio-Nano Measurement and Manufacturing, Zhongshan Institute of Changchun University of Science and Technology, Zhongshan, 528437, China; Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing, Changchun University of Science and Technology, Changchun, 130022, China.

Experimental Cell Research

|May 7, 2026

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This study reveals distinct timing for cell surface changes and mechanical reinforcement during degranulation, offering new biomarkers for allergic diseases. Understanding these biophysical mechanisms aids therapeutic development.

Area of Science:

Cellular Biophysics
Immunology
Nanotechnology

Background:

Degranulation mechanisms in allergic diseases require understanding membrane and cytoskeletal dynamics.
Spatiotemporal coordination during degranulation is incompletely understood.

Purpose of the Study:

To investigate the biophysical mechanisms of degranulation using time-resolved Atomic Force Microscopy.
To quantify phase-specific changes in cell morphology, adhesion, and stiffness during RBL-2H3 cell activation.

Main Methods:

Atomic Force Microscopy (AFM) for time-resolved cell surface mapping.
Quantification of cell height, surface adhesion, and cortical stiffness (Young's modulus).
Characterization of extracellular vesicle (EV) ultrastructure.

Keywords:

Atomic force microscopy Cell degranulation Cell mechanical properties RBL-2H3 cells

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Last Updated: May 9, 2026

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts

Published on: August 27, 2012

Traction Force Microscopy to Study B Lymphocyte Activation

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Main Results:

Cell height and surface adhesion peaked at 8 hours, indicating membrane ruffling and sensitization.
Young's modulus peaked at 12 hours, showing delayed cytoskeletal reinforcement for granule transport.
Decoupled initial membrane sensitization from intracellular execution phase.

Conclusions:

Identified novel nanomechanical biomarkers for degranulation.
Findings aid understanding of vesicle-mediated communication.
Potential to guide stage-specific therapeutic interventions for allergic diseases.