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Updated: May 9, 2026

Separation of Avian Preovulatory Follicle Granulosa and Theca Cell Layers for Downstream Applications
Published on: October 25, 2024
Dynamic expression pattern and regulatory network of circRNAs during chicken ovarian development
Yihan Liu1, Zhiyuan An1, Jingqi Wang1
1College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
None:
Circular RNAs (circRNAs) exert crucial functions in mammalian reproduction; however, their regulation underlying chicken reproduction remains unclear. Here, time-course transcriptome profiles of circRNAs were constructed in chicken ovarian tissues across the entire egg-laying cycle (15 W, 20 W, 30 W, 70 W, 90 W). A total of 6551 circRNAs were identified, and most were derived from exon-exon backsplicing with 400-600 nt. Of these, 1556 circRNAs harbour translation potential in both IRES- and m6A modification-dependent manners. The 1894 differentially expressed circRNAs (DE-circRNAs) exhibited stage-specific expression patterns, and were clustered into 6 significantly co-expressed subclusters and enriched in multiple signaling pathways involving follicular development. Weighted gene co-expression network analysis detected 1016 hub circRNAs, of which 136 circRNAs were co-expressed DE-circRNAs and considered as key candidates regulating chicken ovarian development. These key circRNAs may post-transcriptionally regulate folliculogenesis-related gene via competitive endogenous RNA mechanism. This study reveals potential regulatory roles of circRNAs in chicken ovarian development and egg-laying performance.
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