Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Telomeres and Telomerase

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.

Valence Bond Theory

Valence Bond Theory

Coordination compounds and complexes exhibit different colors, geometries, and magnetic behavior, depending on the metal atom/ion and ligands from which they are composed. In an attempt to explain the bonding and structure of coordination complexes, Linus Pauling proposed the valence bond theory, or VBT, using the concepts of hybridization and the overlapping of the atomic orbitals. According to VBT, the central metal atom or ion (Lewis acid) hybridizes to provide empty orbitals of suitable...

Single-Strand DNA Binding Proteins

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...

Condensins

Condensins

Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...

DNA Topoisomerases

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. Type I...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dual Functional Potential of Poly(ADP-Ribose) Polymerase Inhibitor VIII: A Promising G-Quadruplex Stabilizer Targeting the Promoter of Oncogenes.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026

Same author

Three-State Unfolding of Telomeric G-Quadruplexes through Conformational Switching in Crowded Cell-like Conditions.

Biochemistry·2026

Same author

Design and synthesis of ionic tetraphenylbuta-1,3-diene derivatives: photophysical properties and their suitability in biological environments.

Journal of materials chemistry. B·2025

Same author

Insight into the role of the chemical nature and length of the loop nucleobases in the folding of G-quadruplex by the antimalarial drugs at the DNA interface.

Biointerphases·2025

Same author

Nucleobase Level Information into the Folding of G-Quadruplex by Anti-inflammatory Drugs in the Absence of Salt.

The journal of physical chemistry. B·2025

Same author

Flanking Effect on the Folding of Telomeric DNA Sequences into G-Quadruplex Induced by Antimalarial Drugs.

The journal of physical chemistry. B·2025

Search research articles

Related Experiment Video

Updated: May 9, 2026

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Opposite Charges, Different Stability: Telomeric G-Quadruplexes in Nanoconfinement.

Trideep Majumdar¹, Asim Bisoi¹, Prashant Chandra Singh¹

¹School of Chemical Science, Indian Association for the Cultivation of Science, Jadavpur, Kolkata, India 700032.

The Journal of Physical Chemistry Letters

|May 7, 2026

Summary

Confinement

More Related Videos

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Published on: May 12, 2023

Related Experiment Videos

Last Updated: May 9, 2026

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Published on: May 12, 2023

Area of Science:

Biochemistry
Nanotechnology
Molecular Biology

Background:

Telomere G-quadruplex (G4) structures are crucial for genomic stability.
Understanding G4 folding and stability in confined environments is vital for cellular processes.

Purpose of the Study:

To investigate how the chemical nature of confinement influences telomere G-quadruplex (G4) folding and thermal stability.
To compare the effects of anionic and cationic nanosized water pools on G4 formation and stability.

Main Methods:

Studied telomeric DNA sequences with varying thymine loop arrangements.
Analyzed G4 folding patterns and thermal stability in anionic and cationic nanosized water pools.

Main Results:

Both anionic and cationic water pools induced G4 formation with the same topology.
G4 structures exhibited significantly lower thermal stability in cationic water pools compared to anionic ones.
G4 topology was insensitive to confinement type, but thermal stability was highly dependent on it.

Conclusions:

The chemical nature of confinement significantly impacts the thermal stability of folded telomeric G4.
Differences in interfacial water orientation and hydrogen bonding within cationic vs. anionic pools likely explain stability variations.
Findings offer insights into telomere G4 behavior within confined cellular systems.