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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

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Related Experiment Video

Updated: May 9, 2026

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
10:07

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Published on: March 16, 2022

CRISPR-based technologies for large DNA insertions.

Mary Gracen A Fuller1, Matthew Foley2, Rodolphe Barrangou1

  • 1Genetics and Genomics Program, College of Agricultural and Life Sciences, North Carolina State University, Raleigh, NC, USA; Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC, USA.

Trends in Biotechnology
|May 7, 2026
PubMed
Summary
This summary is machine-generated.

New CRISPR tools enable large-scale DNA editing for medicine and agriculture. These advanced genome editing technologies expand possibilities beyond simple mutations, paving the way for innovative therapies and crop improvements.

Keywords:
CRISPR-Caslarge DNA insertionmobile genetic elementssite-specific recombinase

More Related Videos

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems
10:52

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

Published on: October 14, 2025

Related Experiment Videos

Last Updated: May 9, 2026

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
10:07

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Published on: March 16, 2022

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems
10:52

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

Published on: October 14, 2025

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetics

Background:

  • CRISPR-Cas9 and Cas12 revolutionized precise DNA mutation generation.
  • Current methods are limited to small-scale mutagenesis via DNA nicks and double-stranded breaks (DSBs).
  • There is a growing need for advanced genome editing tools capable of large-scale DNA manipulations.

Purpose of the Study:

  • To review emerging CRISPR-based technologies for large-scale DNA manipulation.
  • To highlight advanced tools that expand payload options and enable DSB-free editing.
  • To discuss the translational potential of these technologies in medicine and agriculture.

Main Methods:

  • Exploration of CRISPR-associated transposons and site-specific recombinases for larger DNA integrations.
  • Analysis of sophisticated combinations like PASTE, PASSIGE, and PrimeRoot.
  • Review of DSB-free editing modalities and expanded payload capacities.

Main Results:

  • Emerging effectors enable larger DNA integrations by combining Cas enzymes with other functions.
  • Advanced combinations (PASTE, PASSIGE, PrimeRoot) offer expanded payload options.
  • New modalities facilitate DSB-free editing, broadening genome editing applications.

Conclusions:

  • Sophisticated CRISPR-based tools are advancing genome editing beyond local mutagenesis.
  • These technologies hold significant translational potential for gene and cell therapies in personalized medicine.
  • The development of these tools is crucial for next-generation crop breeding in sustainable agriculture.