Chromosome-level haplotype-resolved genome assembly of kiwifruit (Actinidia eriantha)

Kiwifruit (Actinidia spp.) is an economically important plant that has undergone rapid development in the 20th century. Among these species, A. eriantha holds considerable agronomic and evolutionary significance. Here, we present a haplotype-resolved, chromosome-level genome assembly for a wild A. eriantha individual. The assembled genome sizes were 663.76 Mb for HAP1 and 633.05 Mb for HAP2, with contig N50 values of 21.82 Mb and 21.39 Mb, respectively. A total of 95.9% of HAP1 (636.55 Mb) and 99.25% of HAP2 (628.28 Mb) sequences were successfully anchored to 29 pseudochromosomes, resulting in scaffold N50 values of 21.89 Mb and 21.50 Mb, respectively. We annotated a total of 39,983 and 40,099 high-confidence protein-coding genes for two haplotypes, respectively. Genome evaluations showed high completeness and accuracy, with BUSCO completeness of 99.5% for both haplotypes as well as QV of 43.11 for HAP1 and 43.42 for HAP2. This high-quality genome provides a valuable reference for comparative and population genomics studies, and will facilitate the identification of genes controlling key agronomic traits, thereby accelerating molecular breeding in kiwifruit.