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Related Experiment Video

Updated: May 9, 2026

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
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In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

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Tracking of Transplanted Stem Cells in Mouse Models Using Practical Fluorescence-Based Protocols.

Riho Kanai1, Arvind Hariharan1, Janaki Iyer1

  • 1Laboratory of Craniofacial Tissue Engineering and Stem Cells, Faculty of Dental Medicine and Oral Health Sciences, McGill University, Montreal, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|May 7, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a dual cell-tracking workflow using PKH26 and GFP labeling for monitoring transplanted cells in mice. These methods enable robust short-term and long-term evaluation of cell engraftment and behavior in regenerative medicine research.

Keywords:
Allogeneic transplantationDirect fluorescent labelingIndirect genetic labelingStem cell trackingXenotransplantation

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Last Updated: May 9, 2026

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Area of Science:

  • Regenerative Medicine
  • Cell Biology
  • Animal Models

Background:

  • Accurate tracking of transplanted cells is crucial for assessing therapeutic efficacy in regenerative medicine.
  • Existing methods may have limitations in duration or stability for comprehensive cell behavior analysis.

Purpose of the Study:

  • To present a practical and versatile cell-tracking workflow for monitoring transplanted cells in mouse tissues.
  • To detail complementary methods for both short-term and long-term cell tracing.

Main Methods:

  • Direct fluorescent labeling using PKH26 for cell membrane tracking.
  • Indirect genetic labeling using Green Fluorescent Protein (GFP) for stable, heritable cell tracking.
  • Optimization of protocols and troubleshooting for both labeling techniques.

Main Results:

  • PKH26 offers rapid, robust membrane labeling suitable for short-term cell migration and distribution studies.
  • GFP provides stable, heritable fluorescence for long-term monitoring of cell persistence and survival.
  • The combined workflow provides a flexible framework for comprehensive cell behavior analysis.

Conclusions:

  • The presented PKH26 and GFP labeling workflow offers a reliable and adaptable approach for evaluating transplanted cell fate in mouse models.
  • These complementary methods enhance the understanding of cell engraftment, survival, distribution, and behavior in transplantation research.
  • This workflow is essential for advancing research in transplantation and regenerative medicine.