NeuroPred-GMC: a dual-branch deep learning architecture for neuropeptide prediction based on gated dilated...

Neuropeptides are multifunctional signaling molecules in the nervous system. By modulating synaptic transmission and integrating physiological systems, they influence a broad range of functions from pain perception to emotional regulation. Predicting neuropeptides can rapidly expand the library of potential therapeutic targets, thereby providing novel candidate molecules for drug development in areas such as analgesics, anti-anxiety medications, and weight-loss drugs. Traditional experimental methods are extremely time-consuming, labor-intensive, these promising alternative computational methods have emerged. In this study, a dual-branch deep learning architecture for neuropeptide prediction known as NeuroPred-GMC are built up based on gated dilated convolutional network with ESM-2 feature representation and multi-scale convolutional network with Prot-T5 feature representation. Dilated convolution exponentially enlarges the receptive field via increased dilation rates, gating mechanism enables dynamic, selective feature enhancement and noise suppression, and multi-scale convolution captures multi-level contextual information. On the independence test set, the accuracy of 93.24%, Sn of 93.69%, Sp of 92.79%, Pre of 92.86%, MCC of 0.8649 and the auROC of 0.9667 are obtained. The experimental results through cross-validation and independent test demonstrate that the proposed model has good robustness and generalizability, and can serve as a supplemental candidate predictor. The source datasets and codes can be freely available at https://github.com/yunyunliang88/NeuroPred-GMC .