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Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
The Phosphorus Cycle01:21

The Phosphorus Cycle

Unlike carbon, water, and nitrogen, phosphorus is not present in the atmosphere as a gas. Instead, most phosphorus in the ecosystem exists as compounds, such as phosphate ions (PO43-), found in soil, water, sediment and rocks. Phosphorus is often a limiting nutrient (i.e., in short supply). Consequently, phosphorus is added to most agricultural fertilizers, which can cause environmental problems related to runoff in aquatic ecosystems.

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Related Experiment Video

Updated: May 9, 2026

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
06:08

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Published on: December 27, 2018

Kinetic-Programmed Hydrolysis Enables Intelligent Time-Evolving Phosphorescence in Water.

Kang Shao1, Haoru Wen1, Wuyan Xie1

  • 1College of Chemical Engineering, Zhejiang University of Technology, Hangzhou, P. R. China.

Advanced Materials (Deerfield Beach, Fla.)
|May 8, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to create programmable aqueous room-temperature phosphorescent (RTP) materials. This breakthrough enables dynamic color changes in nanomaterials for advanced applications like bioimaging and anti-counterfeiting.

Keywords:
carbon dots (CDs)kinetic‐programmed hydrolysisroom‐temperature phosphorescence (RTP)time‐dependent phosphorescence (TDP)

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Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
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Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
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Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
10:38

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

Area of Science:

  • Materials Science
  • Nanotechnology
  • Photochemistry

Background:

  • Developing aqueous room-temperature phosphorescent (RTP) materials with controllable afterglow is challenging.
  • Existing methods often lack dynamic programmability or stability in aqueous environments.

Purpose of the Study:

  • To introduce a universal synthesis paradigm for programmable aqueous RTP materials.
  • To achieve dynamic control over phosphorescence properties for novel applications.

Main Methods:

  • Orchestrating aminosilane hydrolysis kinetics to synthesize silylated carbon dots (Si-CDs).
  • Covalently locking dual-emission centers within a rigid silica matrix.
  • Utilizing aminosilane as a multifunctional building block (carbon source, electron donor, molecular bridge).

Main Results:

  • Synthesized ultra-small nanoparticles (7-9 nm) with exceptional aqueous RTP performance (859 ms lifetime, 29.3% quantum yield).
  • Pioneered programmable time-dependent phosphorescence (TDP) with dynamic color evolution (red to blue).
  • Demonstrated potential in autofluorescence-free in vivo bioimaging, anti-counterfeiting, and 3D data encryption.

Conclusions:

  • The developed synthesis paradigm offers a versatile platform for designing intelligent photonic nanomaterials.
  • Programmable TDP via kinetic control opens new avenues for optical information security.
  • The Si-CDs exhibit significant potential for advanced bioimaging and anti-counterfeiting technologies.