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Updated: May 9, 2026
11:13
Identification of Kinase-substrate Pairs Using High Throughput Screening
Published on: August 29, 2015
Proteomic approaches for interrogating kinase signaling networks
Christine A Berryhill1, Michael P East2, Jocelyne N Hanquier1
1Herman B Wells Center for Pediatric Research, Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana, USA.
The Journal of Investigative Dermatology
|May 8, 2026
Summary
Proteomics methods like MIB-MS and IS-PRM SureQuant analyze kinase activity in skin diseases. These techniques offer scalable strategies for target discovery and therapeutic development in dermatology.
Area of Science:
- Biochemistry and Molecular Biology
- Dermatology
- Proteomics
Background:
- Kinases regulate crucial cellular processes like growth and differentiation.
- Dysregulated kinase activity is implicated in skin diseases such as melanoma and dermatitis.
- Adaptive kinase responses can drive treatment resistance.
Purpose of the Study:
- To review two complementary mass spectrometry-based proteomics methods for functional kinome analysis in dermatology.
- To highlight the application of these methods for understanding kinase networks in skin biology.
- To inform target discovery, biomarker development, and therapeutic strategies.
Main Methods:
- Multiplexed inhibitor beads coupled with mass spectrometry (MIB-MS) enriches for active kinases.
- Internal standard triggered-parallel reaction monitoring (IS-PRM)-targeted proteomics quantifies specific kinase peptides.
- Thermo SureQuant acquisition method enables sensitive quantification from clinical specimens.
Main Results:
- MIB-MS provides unbiased, pathway-level insights into kinase network dynamics and drug specificity.
- IS-PRM SureQuant offers sensitive and consistent quantification of kinase peptides from limited samples, including FFPE.
- Optimized workflows, instrument parameters, and sample requirements are summarized.
Conclusions:
- MIB-MS and IS-PRM SureQuant are orthogonal, scalable strategies for kinome profiling in skin.
- These proteomics methods can advance understanding of skin biology and disease mechanisms.
- The techniques support rational therapeutic strategies and biomarker development in dermatology.
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