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Related Concept Videos

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...

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Related Experiment Video

Updated: May 9, 2026

Identification of Kinase-substrate Pairs Using High Throughput Screening
11:13

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

Proteomic approaches for interrogating kinase signaling networks.

Christine A Berryhill1, Michael P East2, Jocelyne N Hanquier1

  • 1Herman B Wells Center for Pediatric Research, Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana, USA.

The Journal of Investigative Dermatology
|May 8, 2026
PubMed
Summary

Proteomics methods like MIB-MS and IS-PRM SureQuant analyze kinase activity in skin diseases. These techniques offer scalable strategies for target discovery and therapeutic development in dermatology.

Keywords:
Adaptive resistanceChemical proteomicsKinase inhibitorKinome profilingTargeted proteomics

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Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
11:23

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Published on: June 30, 2019

Related Experiment Videos

Last Updated: May 9, 2026

Identification of Kinase-substrate Pairs Using High Throughput Screening
11:13

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
11:23

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Published on: June 30, 2019

Area of Science:

  • Biochemistry and Molecular Biology
  • Dermatology
  • Proteomics

Background:

  • Kinases regulate crucial cellular processes like growth and differentiation.
  • Dysregulated kinase activity is implicated in skin diseases such as melanoma and dermatitis.
  • Adaptive kinase responses can drive treatment resistance.

Purpose of the Study:

  • To review two complementary mass spectrometry-based proteomics methods for functional kinome analysis in dermatology.
  • To highlight the application of these methods for understanding kinase networks in skin biology.
  • To inform target discovery, biomarker development, and therapeutic strategies.

Main Methods:

  • Multiplexed inhibitor beads coupled with mass spectrometry (MIB-MS) enriches for active kinases.
  • Internal standard triggered-parallel reaction monitoring (IS-PRM)-targeted proteomics quantifies specific kinase peptides.
  • Thermo SureQuant acquisition method enables sensitive quantification from clinical specimens.

Main Results:

  • MIB-MS provides unbiased, pathway-level insights into kinase network dynamics and drug specificity.
  • IS-PRM SureQuant offers sensitive and consistent quantification of kinase peptides from limited samples, including FFPE.
  • Optimized workflows, instrument parameters, and sample requirements are summarized.

Conclusions:

  • MIB-MS and IS-PRM SureQuant are orthogonal, scalable strategies for kinome profiling in skin.
  • These proteomics methods can advance understanding of skin biology and disease mechanisms.
  • The techniques support rational therapeutic strategies and biomarker development in dermatology.