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miR-135a-5p exerts tumor-suppressive effects in 769P renal carcinoma cells through the TFAP2A/KRT8 signaling axis

Delong Xie1, Jizhe Zhou1, Yueyue Guo1

  • 1College of Basic Medical Sciences, Youjiang Medical University for Nationalities, Baise, Guangxi, China.

In Vitro Cellular & Developmental Biology. Animal
|May 8, 2026
PubMed

Insights

MicroRNA miR-135a-5p suppresses clear cell renal cell carcinoma (ccRCC) progression by targeting TFAP2A and KRT8. This finding highlights miR-135a-5p as a potential therapeutic target for ccRCC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) play crucial roles in cancer development.
  • The specific function of miR-135a-5p in clear cell renal cell carcinoma (ccRCC) requires further investigation.

Purpose of the Study:

  • To investigate the role of miR-135a-5p in ccRCC progression.
  • To elucidate the molecular mechanism underlying miR-135a-5p's function in ccRCC.

Main Methods:

  • Weighted gene co-expression network analysis (WGCNA) to identify gene associations.
  • Expression analysis in ccRCC tissues and cell lines.
  • Functional assays to assess cell progression.
  • Luciferase reporter assays to confirm direct binding to TFAP2A 3'UTR.

Main Results:

  • miR-135a-5p expression is downregulated in ccRCC.
  • miR-135a-5p inhibits ccRCC cell line 769P progression.
  • miR-135a-5p directly targets TFAP2A, reducing its mRNA stability.
  • TFAP2A activates KRT8 transcription, mediating miR-135a-5p's inhibitory effect.

Conclusions:

  • miR-135a-5p acts as a tumor suppressor in ccRCC.
  • The miR-135a-5p/TFAP2A/KRT8 axis is a key pathway in ccRCC progression.
  • miR-135a-5p represents a potential therapeutic target for ccRCC.
Keywords:
Clear cell renal cell carcinomaMiR-135a-5pProgressionTFAP2A

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