Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Amplifying Signals via Enzymatic Cascade

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...

Cell Signaling Feedback Loops

Cell Signaling Feedback Loops

Positive and negative feedback loops are crucial for regulating biological signaling systems. These feedback loops are processes that connect output signals to their inputs.
Negative feedback loops
Most signaling systems have negative feedback loops that can perform different functions such as output limiter, and adaptation.
Output limiter
Upon receiving an input signal, the cellular response rapidly increases until a threshold is reached. Beyond this threshold, a negative feedback loop...

Calmodulin-dependent Signaling

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

Assembly of Signaling Complexes

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...

IP3/DAG Signaling Pathway

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...

Amplifying Signals via Second Messengers

Amplifying Signals via Second Messengers

Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Weight-Controllable Biobarcode Probes for Multi-input Breast Cancer Diagnosis.

Nano letters·2026

Same author

Reagent-Free Molecular Pendulum Biosensor with Antibody-Aptamer Dual Recognition for Protein Analysis.

ACS sensors·2026

Same author

CEMs-SELEX: DNAzyme-powered de novo biomarker discovery and noninvasive cancer diagnosis.

Science advances·2026

Same author

Chemically modified nucleic acid nanodevices: molecular engineering for drug delivery, theranostics, biosensing and diagnostics.

Chemical communications (Cambridge, England)·2026

Same author

Targeting Integrin β3 in Endothelial Cells Attenuates Cardiac Fibrosis Through the Calcium-Calmodulin-Dependent Protein Kinase IIα- cAMP-Responsive Element Binding Protein 1 Signaling Axis in Pressure Overload-Induced Heart Failure.

Journal of the American Heart Association·2026

Same author

Identifying a cancer therapeutic target: Cell-SELEX identifies a membrane protein for aptamer-mediated growth suppression.

Proceedings of the National Academy of Sciences of the United States of America·2026

Search research articles

Related Experiment Video

Updated: May 10, 2026

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

Proximity-Induced Phosphatase-Recruiting DNA Feedback Switch for Self-Adaptive Modulation of Receptor Signaling and

Yuchen Wu¹, Guanyu Yin¹, Qin Zhang¹

¹State Key Laboratory of Chemo and Biosensing, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Changsha 410082, China.

Journal of the American Chemical Society

|May 8, 2026

Summary

Scientists created a DNA feedback switch that controls cell signaling by sensing receptor proximity. This bioinspired nanomedicine platform precisely regulates cell behavior and offers potential for adaptive therapies.

More Related Videos

Development and Application of Rapamycin-regulated Tyrosine Phosphatases

Development and Application of Rapamycin-regulated Tyrosine Phosphatases

Published on: September 6, 2024

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

Related Experiment Videos

Last Updated: May 10, 2026

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

Development and Application of Rapamycin-regulated Tyrosine Phosphatases

Development and Application of Rapamycin-regulated Tyrosine Phosphatases

Published on: September 6, 2024

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

Area of Science:

Biotechnology
Nanomedicine
Molecular Biology

Background:

Transmembrane receptors are crucial for cell signaling, tissue homeostasis, and disease.
Current methods struggle to control cellular processes based on spatial cues like molecular proximity.
Dysregulated receptor signaling contributes to disease initiation and progression.

Purpose of the Study:

To develop a novel system for precise and self-adaptive modulation of transmembrane receptor signaling.
To emulate natural receptor feedback regulation using a proximity-induced mechanism.
To create a bioinspired nanomedicine platform for controlling cellular processes.

Main Methods:

Developed a proximity-induced phosphatase-recruiting DNA feedback switch (PPDFS).
Utilized DNA nanostructures and aptamer-mediated molecular recognition for sensor design.
Engineered PPDFS to detect receptor dimerization and recruit the phosphatase PTPRF.

Main Results:

PPDFS sensitively detects cell surface receptor dimerization.
Receptor proximity triggers both signaling activation and PPDFS-mediated inhibition, maintaining homeostasis.
Demonstrated PPDFS regulation of epithelial-mesenchymal transition (EMT), cytoskeletal changes, and cell migration.

Conclusions:

PPDFS provides specific and self-adaptive control over transmembrane receptor signaling.
The system elucidates the signaling cascade from receptor activity to cellular responses like EMT.
PPDFS represents a versatile nanomedicine platform adaptable to various receptor systems for precise therapeutic control.