Rearing, dissection, and temporal transcriptomic profiling protocols to study density-dependent phenotypic plasticity...

Here, we present a protocol for generating gregarious and solitarious density-dependent phenotypes in multiple Schistocerca locust and grasshopper species under controlled environmental conditions. We describe cage setup, feeding, animal handling, and sterile dissection workflows to isolate nervous, chemosensory, gut, fat body, and female reproductive tissues from nymphs and adults. This protocol emphasizes rapid tissue stabilization and RNase-control practices for downstream single-tissue DNA and RNA analyses.