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Related Experiment Video

Updated: May 10, 2026

Mass-Rearing and Molecular Studies in Tortricidae Pest Insects
06:22

Mass-Rearing and Molecular Studies in Tortricidae Pest Insects

Published on: March 25, 2022

Rearing, dissection, and temporal transcriptomic profiling protocols to study density-dependent phenotypic plasticity

Maéva A Techer1, Vivian A Peralta Santana2, Brandon Woo1

  • 1Texas A&M University, Department of Entomology, College Station, TX 77843, USA.

STAR Protocols
|May 8, 2026
PubMed
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This study details a protocol for inducing locust and grasshopper phenotypes and isolating tissues for genetic analysis. It ensures rapid stabilization and RNA preservation for DNA and RNA studies.

Area of Science:

  • Entomology
  • Animal Behavior
  • Molecular Biology

Background:

  • Locust and grasshopper phenotypes are influenced by environmental factors, particularly density.
  • Understanding the genetic basis of these density-dependent phenotypes requires robust tissue isolation techniques.

Purpose of the Study:

  • To present a standardized protocol for generating gregarious and solitarious phenotypes in locusts and grasshoppers.
  • To outline sterile dissection workflows for isolating specific tissues for molecular analyses.
  • To emphasize techniques for rapid tissue stabilization and RNase control.

Main Methods:

  • Controlled environmental conditions to induce density-dependent phenotypes.
  • Detailed cage setup, feeding, and animal handling procedures.
Keywords:
BehaviorEvolutionary biologyMolecular Biology

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  • Sterile dissection techniques for nervous, chemosensory, gut, fat body, and reproductive tissues.
  • Rapid tissue stabilization and RNase inactivation methods.

    • Main Results:

    • Successful generation of gregarious and solitarious phenotypes across multiple Schistocerca species.
    • Demonstrated isolation of various tissues from both nymphal and adult stages.
    • Established practices for preserving RNA integrity during tissue processing.

    Conclusions:

    • The presented protocol provides a reliable method for studying density-dependent phenotypes in locusts and grasshoppers.
    • This standardized approach facilitates downstream DNA and RNA analyses of specific tissues.
    • The protocol supports research into the molecular mechanisms underlying locust phase polyphenism.