Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 10, 2026

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae

Published on: March 7, 2025

AOP-Guided Toxicogenomic Mapping Profiles for Mechanism-Informed Toxicity Pattern Prediction in Zebrafish.

Tianle Fan¹, Aoran Gu¹, Wenqi Xu¹

¹State Key Laboratory of Water Pollution Control and Green Resource Recycling, School of the Environment, Nanjing University, Nanjing, Jiangsu 210023, China.

Environmental Science & Technology

|May 8, 2026

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A unified analysis of global riverine eDNA reveals common associations of fish biodiversity with drainage characteristics.

Nature ecology & evolution·2026

Same author

From Silent Spring to the Awakening of AI: 60 Years of Ecotoxicology Research in <i>ES&T</i>.

Environmental science & technology·2026

Same author

Precipitation Modulates the Impact of Human Activities on Riverine Food Webs Revealed by Environmental DNA.

Ecology and evolution·2026

Same author

Integrated Reanalysis of Global Riverine Fish eDNA Datasets Shows Robustness and Congruence of Biodiversity Conclusions.

Molecular ecology·2026

Same author

Geographic Patterns of Intra- and Interspecific Diversity of Riverine Fish Species in the Italian Northern Apennines and Ligurian Alps.

Ecology and evolution·2026

Same author

Network-Based Assessment of Environmental Neurotoxicants Using High-Throughput Transcriptomics in Zebrafish Embryos.

Environmental science & technology·2026

This summary is machine-generated.

We developed a new framework, adverse outcome pathway (AOP)-guided chemical toxicogenomic mapping profiles (AOP-CTMP), to efficiently identify environmental pollutant effects. This method accurately predicts toxicity patterns, streamlining chemical safety assessments.

Area of Science:

Environmental Toxicology
Computational Biology
Genomics

Background:

Environmental pollutants often exhibit complex, non-specific biological effects, necessitating extensive bioassays for toxicity screening.
Traditional toxicity testing methods are laborious, time-consuming, and may not capture the full spectrum of pollutant impacts.

Purpose of the Study:

To develop a novel framework, AOP-CTMP, for identifying key effects of environmental pollutants.
To integrate known Adverse Outcome Pathways (AOPs) for aquatic vertebrates into a predictive toxicogenomic mapping system.
To enable efficient and accurate prediction of chemical toxicity patterns.

Main Methods:

Integrated 15 recognized AOPs for aquatic vertebrates into comprehensive AOP networks.
Generated two-dimensional gene matrices and mapped chemical perturbations to create AOP-CTMPs.

Keywords:

adverse outcome pathway high throughput new approach methodology toxicogenomics

More Related Videos

Zebrafish Larvae as a Model to Evaluate Potential Radiosensitizers or Protectors

Zebrafish Larvae as a Model to Evaluate Potential Radiosensitizers or Protectors

Published on: August 25, 2022

Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos

Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos

Published on: August 25, 2019

Related Experiment Videos

Last Updated: May 10, 2026

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae

Published on: March 7, 2025

Zebrafish Larvae as a Model to Evaluate Potential Radiosensitizers or Protectors

Zebrafish Larvae as a Model to Evaluate Potential Radiosensitizers or Protectors

Published on: August 25, 2022

Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos

Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos

Published on: August 25, 2019

Utilized a database of 566 compounds, their genomic perturbations, key events, and adverse effects.

Employed a multilayer perceptron model for prediction, achieving high accuracy (AUC = 0.89-0.98).

Main Results:

The AOP-CTMP framework successfully predicted primary effects of environmental pollutants.
Predictions for 13 representative chemicals were validated through in vitro and in vivo experiments.
Predicted individual-level effects demonstrated strong consistency with observed toxicity patterns.
The multilayer perceptron model showed excellent performance and high accuracy in internal validation.

Conclusions:

AOP-CTMP offers a novel, systematic strategy for identifying key effects during toxicity screening.
The framework enables reliable prediction of toxicity patterns from chemical perturbations.
This approach has significant practical potential for advancing environmental toxicology and chemical safety assessment.