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Updated: May 10, 2026

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Published on: March 9, 2010

Tailored interfacial modulation for constructing high-affinity imprinted polymers for protein isolation in complex

Xinya Xu¹, Yanhui Ge², Lu Wang³

¹College of Pharmacy, Shaanxi University of Chinese Medicine, Xi'an, 712046, China; Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine, Xianyang, 712046, China.

Journal of Chromatography. A

|May 8, 2026

Summary

Researchers developed high-performance lysozyme-imprinted polymers using interfacial modulation. This strategy enables efficient protein separation from complex biological samples with high selectivity and capacity.

Keywords:

Dual-monomer imprinting Interfacial engineering Lysozyme selective separation Magnetic biochar Protein recognition

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Last Updated: May 10, 2026

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

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Published on: March 9, 2010

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Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

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Published on: May 5, 2017

Area of Science:

Separation Science
Polymer Chemistry
Biochemistry

Background:

Precise recognition of high-value proteins from complex biological matrices is a significant challenge.
Existing protein separation methods often lack specificity and efficiency.

Purpose of the Study:

To develop a tailored interfacial modulation strategy for constructing high-performance lysozyme-imprinted polymers.
To enhance the stability and specificity of recognition sites for efficient protein separation.

Main Methods:

Interfacial microenvironment regulation combined with a dual-monomer approach.
Synthesis of lysozyme-imprinted polymers.
Evaluation of adsorption capacity, imprinting factor, selectivity, and stability.

Main Results:

Polymers demonstrated high adsorption capacity (395.13 mg/g) and imprinting factor (8.78).
Equilibrium was reached rapidly (within 30 min).
High selectivity for lysozyme over competing proteins and stable performance through adsorption-desorption cycles were observed.

Conclusions:

The developed strategy effectively modulates the interfacial microenvironment for efficient protein separation.
The high-performance lysozyme-imprinted polymers show practical applicability in isolating proteins from real biological matrices.
This work offers a stepwise approach for advanced protein separation technologies.