Related Experiment Video
Updated: May 10, 2026
Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
Published on: August 22, 2019
REAPing the benefits of comprehensive specificity profiling for therapeutic antibody development
Luke M Tomasovic1, Sofia Farhangnia2, Jamie B Spangler3
1Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Translational Therapeutics and Regenerative Engineering Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Abstract:
In this issue of Structure, Dai et al.1 systematically profile off-target reactivity across 174 clinical-stage and approved antibody therapeutics, revealing that 28% bind unintended human extracellular proteins. They validate functionally relevant off-target interactions and show that antibody engineering can eliminate these liabilities while maintaining target affinity and favorable developability properties.
