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Updated: May 10, 2026

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
Published on: June 26, 2013
Let there be light: Targeted fluorescent labels illuminate cryo-FIB/ET of synapses
Spencer J Rothfuss1, Qiangjun Zhou2
1Brain Institute, Vanderbilt University, Nashville, TN 37240-7933, USA; Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37240-7933, USA; Chemical and Physical Biology Program, Vanderbilt University, Nashville, TN 37240-7933, USA.
Abstract:
Focused ion beam milling exposes buried cellular regions, such as chemical synapses within neuronal networks, for high-resolution electron microscopy. In this issue of Structure, Do et al.1 use fluorescent tags to target milling and imaging to synapses, successfully removing confounding material and capturing synaptic ultrastructure in high resolution.

