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Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format

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Published on: December 5, 2025

Extracellular alkalinization boosts plant immunity.

Yuhang Ming¹, Wen Song¹

¹State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China.

|May 8, 2026

Summary

This summary is machine-generated.

Plant immune responses involve extracellular alkalinization, a process regulated by proton pump phosphorylation. This mechanism is vital for effective local defense and priming the plant's broader immune system.

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Identification of Post-translational Modifications of Plant Protein Complexes

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Published on: February 22, 2014

Area of Science:

Plant biology
Molecular immunology
Plant physiology

Background:

Extracellular alkalinization is a key indicator of plant immune activation.
The precise molecular mechanisms controlling this pH shift during plant defense remain incompletely understood.

Purpose of the Study:

To elucidate the regulatory mechanisms underlying extracellular alkalinization in plant immunity.
To investigate the role of proton pump phosphorylation in plant defense responses.

Main Methods:

Phosphoproteomic analysis to identify phosphorylated proteins in plant immune signaling.
Biochemical assays to confirm the activity of proton pumps upon phosphorylation.
Genetic studies in plants to assess the impact of altered proton pump phosphorylation on defense.

Main Results:

Extracellular alkalinization is directly regulated by the phosphorylation status of key proton pumps.
Phosphorylation enhances proton pump activity, leading to the observed extracellular pH changes.
Disruption of this phosphorylation-dependent regulation compromises both local plant immunity and systemic immune priming.

Conclusions:

Proton pump phosphorylation is a critical regulatory node in plant extracellular alkalinization during immunity.
This mechanism is essential for mounting effective localized defenses and establishing long-term systemic protection in plants.