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Updated: May 10, 2026
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Design and Validation of a Volumetric-extrusion Bioprinter for Bioprinting of Soluble Basement Membrane Extract for Translational Research
Published on: March 28, 2025
Novel printing strategy for high fidelity extrusion-based bioprinted multi-material nested models.
Maialen Zelaia Amilibia1,2, Uxue Aizarna-Lopetegui3, Laura Pérez Sánchez1
1Digital Health and Biomedical Technologies, Vicomtech Foundation, Basque Research and Technology Alliance (BRTA), Donostia-San Sebastian, Spain.
Biomedical Materials (Bristol, England)
|May 8, 2026
Summary
This study introduces a new bioprinting protocol for creating precise 3D blood vessel models. The method enhances trajectory generation, improving structural integrity and reducing printing time for tissue engineering applications.
Area of Science:
- Biomedical Engineering
- Tissue Engineering
- 3D Bioprinting
Background:
- Precise 3D-printed multilayered blood vessel biomodels are crucial for drug development and tissue engineering.
- Existing extrusion-based bioprinting methods often lack geometric fidelity in cylindrical constructs.
Purpose of the Study:
- To develop a novel printing protocol for extrusion-based multi-material nested cylindrical model bioprinting.
- To enhance trajectory generation for improved geometric fidelity and fabrication efficiency.
Main Methods:
- Developed Computer-Aided Design and Computer-Aided Manufacturing (CAD/CAM) software for multi-material nested cylindrical models.
- Implemented an innovative printing protocol modifying layer start points, optimizing printing order, and adjusting photopolymerization.
- Tested the protocol on three multi-material tissue models and compared with BIOCAD software.
Main Results:
- The new protocol significantly improved cylindrical structure integrity.
- Minimized printing collisions and reduced overall printing time compared to existing methods.
- Demonstrated superior printing fidelity in generated tissue models.
Conclusions:
- The optimized trajectory-generation approach provides a robust framework for creating accurate in vitro vascular models.
- This method can accelerate drug discovery and reduce animal testing in biomedical research.
- Validates the effectiveness for extrusion-based cylindrical bioprinting applications.
