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Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
Published on: September 20, 2016
Sustainable production of natural sweeteners through synthetic biology
Dong Li1, Jinxin Fan1, Jianfei Zong2
1School of Life Sciences, Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan 750021, China.
Abstract:
With the upgrading of global health demands and safety controversies surrounding artificial sweeteners, natural sweeteners have emerged as a research hotspot thanks to their low-calorie content, high safety, and potential physiological activities. Traditional plant extraction methods are limited by bottlenecks such as long growth cycles and geographical dependence, while synthetic biology has provided an innovative pathway for the efficient and sustainable production of natural sweeteners through metabolic engineering and enzyme engineering, by constructing microbial cell factories. This review systematically summarizes the metabolic pathways and microbial synthesis methods of 12 representative natural sweeteners (including sweet proteins, terpenoid glycosides, flavonoids, polyols, and monosaccharides). By analyzing the advantages and disadvantages of different synthetic schemes as well as the core technical bottlenecks in current production processes, and integrating the latest research progress, this review provides theoretical support and technical references for the future industrial optimization and production of natural sweeteners.
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