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Updated: May 10, 2026
RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
Published on: March 11, 2014
Detection of papillomavirus DNA in uterine flushing samples from healthy mares reveals diverse genotypes with
Mariassunta Claudia D'Amato1, Anna Cutarelli2, Edoardo Vigetti3
1Dipartimento di Medicina Veterinaria e delle Produzioni Animali, Università degli Studi di Napoli Federico II, Napoli, Italy.
Abstract:
A molecular study to assess the presence of some papillomaviruses was performed on 105 uterine flushing samples of healthy mares using real time quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR). Bovine, equine, and ovine papillomavirus DNA was detected in 27 uterine flushing samples through dPCR (~ 25.71%); qPCR was able to detect 18 positive samples (~ 17.14%). Differences between the two molecular protocols were significant as shown by McNemar's test (p˂ 0.005). EcPV DNA was detected in 10 samples by dPCR (~ 9.52%) of uterine flushing samples. qPCR detected EcPV DNA in 7 samples (~ 6.66). dPCR revealed OaPV1 DNA in 13 samples (12.38%). qPCR revealed OaPV1 DNA in 9 samples (8.57%). BPV DNA was found by dPCR in 4 samples (~ 3.80%); qPCR revealed BPV DNA in two uterine flushing samples, that is ~ 1.90% of healthy uterine flushing samples. Overall, OaPV1 was the most detected papillomavirus from mare uterus. Similar molecular findings were found in the virobiota of mare vagina. EcPV9 was the most prevalent equine PV genotype. EcPV8, BPV13, ChPV1, ChPV2, OaPV2, OaPV3, and OaPV4 were not detected. Our findings are consistent with non-strict host specificity of BPVs and OaPVs.
