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Updated: May 10, 2026
Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test
Published on: July 14, 2023
Multiplexed immunochromatographic strip based on multicolor fluorescent microspheres for simultaneous detection of
1Shanghai Engineering Research Center for Food Rapid Detection, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, No. 516, Jungong Road, P.O. Box 454, 200093, P. R. China.
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A novel multiplex immunochromatographic assay (ICA) based on quantum-dot-encoded multicolor fluorescent microsphere (MFMs) was developed for the simultaneous and quantitative detection of prometryn (PRO), diquat (DQ), and sulfometuron-methyl (SMR). Red, yellow, and green MFMs were fabricated by embedding CdSe/ZnS QDs into polymer microspheres and subsequently conjugated with specific monoclonal antibodies, generating highly uniform MFM-mAb probes with clear and stable emission signals. The multiplex ICA exhibited excellent analytical performance, with limits of detection of 0.41 ng/mL for PRO, 9.36 ng/mL for DQ, and 1.32 ng/mL for SMR, better than most ELISA-based assays. The strip also demonstrated high specificity, good storage stability, and reliable performance in complex matrices. In spiked river water and vegetable samples, recoveries ranged from 85.53% to 109.67% with RSDs of 1.33-7.02%, indicating strong applicability. Overall, this MFM-ICA provides an effective platform for rapid multi-herbicide screening.
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