Related Experiment Video
Updated: May 10, 2026
08:26
Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
Published on: June 25, 2018
Long non-coding RNA RsLNC2361 positively regulate anthocyanin accumulation in radish
Haiyi Liu1,2, Xiaobo Luo3, Linjun Wu1,2
1College of Agriculture, Guizhou University, Guiyang, Guizhou, 550025, China.
BMC Plant Biology
|May 8, 2026
Summary
Long non-coding RNA (lncRNA) RsLNC2361 positively regulates anthocyanin accumulation in radish. This discovery offers insights into breeding radishes with enhanced anthocyanin content for improved nutritional value.
Area of Science:
- Plant molecular biology
- Genomics
- Biochemistry
Background:
- Radish (Raphanus sativus L.) is a vital Brassicaceae vegetable crop with significant nutritional and medicinal benefits.
- Anthocyanin-rich radish varieties are highly sought after for their vibrant color and health-promoting properties.
- The regulatory role of long non-coding RNA (lncRNA) in anthocyanin accumulation in radish remains underexplored.
Purpose of the Study:
- To investigate the function of the differentially expressed lncRNA RsLNC2361 in regulating anthocyanin biosynthesis in radish.
- To elucidate the molecular mechanism underlying RsLNC2361-mediated anthocyanin accumulation.
Main Methods:
- LncRNA sequencing to identify differentially expressed lncRNAs.
- Gene overexpression and virus-induced gene silencing (VIGS) to assess RsLNC2361 function.
- Transcriptome and metabolome sequencing to analyze gene expression and metabolite profiles.
- Bioinformatic analysis to predict lncRNA-miRNA interactions.
Main Results:
- RsLNC2361 was found to be differentially expressed between red and white skin radish.
- Overexpression of RsLNC2361 promoted anthocyanin accumulation, while silencing decreased it, confirming a positive regulatory role.
- RsLNC2361 modulated the expression of anthocyanin structural genes.
- Integrated transcriptomic and metabolomic analyses revealed enrichment of pathways related to flavonoid and phenylpropanoid biosynthesis.
- miRNA858 was predicted to target RsLNC2361, suggesting a potential competitive endogenous RNA (ceRNA) mechanism.
Conclusions:
- RsLNC2361 plays a crucial role in promoting anthocyanin accumulation in radish.
- This study reveals a novel regulatory pathway involving lncRNA in anthocyanin biosynthesis.
- The findings provide a theoretical foundation for breeding radish varieties with enhanced anthocyanin content.
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
01:20Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
01:29Translational Regulation
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
01:23Transcriptional Regulation: Riboswitches
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
01:25Cell Signaling in Plants
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...