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Updated: May 11, 2026
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
Size-dependent AIENPs enhanced RPA-CRISPR/Cas12a mediated lateral flow assay for ultrasensitive detection of
Yuanqian Xiong1, Shaling Ye1, Yuqian Guo1
1State Key Laboratory of Food Science and Resource, Nanchang University, Nanchang 330047, PR China; School of Food Science and Technology, Nanchang University, Nanchang 330047, PR China.
Abstract:
In this study, we developed a lateral flow assay (LFA) utilizing aggregation-induced emission nanoparticles (AIENPs) integrated with recombinase polymerase amplification (RPA) and CRISPR/Cas12a for the ultrasensitive detection of Staphylococcus aureus (S. aureus). We systematically evaluated the influence of AIENP particle size (250, 350, 450, and 600 nm) on the performance of LFA detection. Notably, the 450 nm AIENPs significantly enhanced the sensitivity of the strips due to their low background signal and size-dependent luminescent property. Under optimal conditions, the AIENPs-LFA demonstrated a 100-fold increase in sensitivity over conventional AuNPs-LFA. Combined with RPA, the assay achieved a remarkably low LOD of 9.95 CFU/mL for S. aureus, with a broad linear range (2.4-2.4 × 104 CFU/mL) and high specificity against other foodborne pathogens. Intra- and inter-assay validations indicated high accuracy (recoveries of 83.13%-106.93%) and precision (RSD < 14.42%) in S. aureus fortified milk samples.
