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Updated: May 11, 2026
Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
Published on: September 17, 2017
Ultrasensitive and selective detection of metronidazole residues in milk powder using a novel 3D MOF fluorescent
Xuanqi Zhang1, Guiling Gao1, Xiaoyong Lai1
1State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering, National Demonstration Center for Experimental Chemistry Education, College of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, China.
Abstract:
The presence of metronidazole (MNZ) residues in food poses significant risks to human health. In this study, two metal-organic framework (MOF)-based fluorescent probes, [Ba(bbda)]n (1) and [Cd(bbda)]n (2), were developed for the rapid and ultrasensitive detection of MNZ in food samples. Upon addition of MNZ, both probes exhibited significant fluorescence quenching with favorable linear ranges and low detection limits of 37 nM and 64 nM, respectively. Fluorescence lifetime and density functional theory (DFT) calculations revealed that the quenching mechanism involves a synergistic effect of the inner filter effect (IFE), photoinduced electron transfer (PET), and specific ligand-analyte interactions. Probe 1 was successfully applied to quantify MNZ in commercial pharmaceutical tablets and milk powder, achieving excellent recoveries of 97% and 102.6%, respectively, demonstrating its reliability for trace-level MNZ detection in food matrices.
Insights
This study introduces novel metal-organic framework (MOF) fluorescent probes for detecting metronidazole (MNZ) residues in food. These probes offer rapid, ultrasensitive detection, ensuring food safety and protecting human health from harmful contaminants.
Area of Science:
- Materials Science
- Analytical Chemistry
- Food Safety
Background:
- Metronidazole (MNZ) residues in food present significant human health risks.
- Accurate and sensitive detection methods are crucial for monitoring MNZ contamination in food products.
Purpose of the Study:
- To develop and validate novel metal-organic framework (MOF)-based fluorescent probes for the rapid and ultrasensitive detection of metronidazole (MNZ) in food samples.
- To investigate the quenching mechanism of the fluorescent probes upon interaction with MNZ.
Main Methods:
- Synthesis and characterization of two MOF-based fluorescent probes: [Ba(bbda)]n (1) and [Cd(bbda)]n (2).
- Fluorescence spectroscopy was used to assess the detection performance of the probes for MNZ.
- Fluorescence lifetime measurements and density functional theory (DFT) calculations were employed to elucidate the quenching mechanism.
- Application of Probe 1 for MNZ quantification in real food samples (pharmaceutical tablets and milk powder).
Main Results:
- Both probes demonstrated significant fluorescence quenching in the presence of MNZ.
- Probe 1 exhibited a low detection limit of 37 nM, and Probe 2 had a detection limit of 64 nM.
- The quenching mechanism was attributed to a synergistic effect of the inner filter effect (IFE), photoinduced electron transfer (PET), and specific ligand-analyte interactions.
- Probe 1 achieved excellent recovery rates (97% and 102.6%) when applied to pharmaceutical tablets and milk powder, respectively.
Conclusions:
- The developed MOF-based fluorescent probes enable rapid and ultrasensitive detection of metronidazole in food matrices.
- Probe 1 demonstrates high reliability for trace-level MNZ detection in complex food samples, contributing to enhanced food safety.
- The mechanistic study provides valuable insights into the probe-analyte interactions for future sensor development.
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