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Acetylated Vps1 initiates autophagy and regulates the sorting nexin dynamics essential for pathogenicity in Fusarium

Xin Chen1, Xiaomin Chen2, Zhenyu Fang1

  • 1State Key Laboratory of Agricultural and Forestry Biosecurity & Key Laboratory of Bio-Pesticide and Chemical Biology, Ministry of Education, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.

Autophagy
|May 10, 2026
PubMed

Insights

This study reveals how histone acetyltransferase FgHat2 regulates autophagy in Fusarium graminearum. Acetylation of FgVps1 by FgHat2 is crucial for initiating autophagy and maintaining cellular homeostasis in this fungus.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Mycology

Background:

  • Macroautophagy/autophagy is vital for cellular homeostasis and fungal pathogenicity.
  • Mechanisms regulating autophagy homeostasis in phytopathogenic fungi are not well understood.

Purpose of the Study:

  • To identify key regulators of autophagy homeostasis in Fusarium graminearum.
  • To elucidate the molecular mechanisms controlling autophagy initiation and regulation.

Main Methods:

  • Genetic analysis of the FgHat2-FgVps1-FgSnx4 module.
  • Biochemical assays to study protein interactions and modifications.
  • Microscopy to track protein trafficking during autophagy.

Main Results:

  • The FgHat2-FgVps1-FgSnx4 module was identified as a critical regulator of autophagy.
  • FgHat2 acetylates FgVps1, promoting its interaction with FgAtg8 and FgSnx4 release.
  • FgSnx4 directs FgAtg9 trafficking and interacts with FgAtg8, ensuring autophagy modulation.

Conclusions:

  • An acetylation-dependent mechanism coordinates autophagy in Fusarium graminearum.
  • This provides new insights into autophagy regulation in phytopathogenic fungi.
Keywords:
AcetylationFusarium graminearumautophagydynamin-like GTPasesorting nexin

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