Related Experiment Video For Bladder Cancer diagnosis
Updated: May 12, 2026
Cell-Free DNA Integrity Analysis in Urine Samples
Published on: January 5, 2017
Enhanced detection of bladder cancer using combined circulating tumor cells, urine-derived epithelial cells, and
Zhijie Jiang1,2, Cheng Yuan3, Honggang Yuan2
1Department of Anesthesiology, Yichang Central People's Hospital, China Three Gorges University, Yichang, 443000, China.
Purpose:
The sensitivity of bladder cancer detection using a single biomarker from single sample type is limited. This study aimed to investigate whether a combined approach utilizing multiple biomarkers from different clinical samples could improve detection sensitivity.
Methods:
A total of 85 patients with bladder cancer and 30 healthy individuals were enrolled in this study. Urine and blood samples were collected for the isolation of urine-derived epithelial cells (UDECs) and circulating tumor cells (CTCs). These cells were then analyzed via PD-L1 assay and fluorescence in situ hybridization (FISH) targeting chromosomes 7 and 8. In parallel, matched urine samples from patients underwent conventional urine exfoliation cytology testing (UEC). All data were analyzed in conjunction with pathological information using specialized statistical software.
Results:
Analysis of CTCs demonstrated a significantly higher bladder cancer detection rate (78.6%) compared to UEC (36.7%). The combination of UDEC-FISH and CTC analysis utilizing urine and blood samples achieved a higher detection rate (94.1%) than the combination of UDEC-FISH with UEC performed on the same urine sample (79.8%). Furthermore, combined analysis of three markers of CTC, UEC, and UDEC-FISH (96.5%) or CTC, UEC, and UDEC-PD-L1 (90.6%) yielded significantly higher detection rates than any single biomarker analysis alone.
Conclusion:
Integrating multiple biomarkers from distinct sample types significantly enhances the detection sensitivity for bladder cancer.
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