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The Pentatricopeptide Repeat Protein OsPPR7 Confers Cold Tolerance at the Booting Stage in Rice by Regulating ABA and
Changsheng Liu1, Jingwen Li1, Yantao Li1
1Key Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region, Ministry of Education, Northeast Agricultural University, Harbin, 150030, China.
We identified OsPPR7 as a key gene for rice cold tolerance. Its mutation causes pollen defects under cold stress, while its proper function ensures reproductive success and climate resilience.
Area of Science:
- Plant Science
- Genetics
- Agronomy
Background:
- Cold stress during the booting stage significantly impairs rice pollen development and grain yield.
- Developing cold-tolerant rice varieties is crucial for global food security.
Purpose of the Study:
- To identify the genetic basis of cold sensitivity in rice reproductive development.
- To elucidate the molecular mechanism of OsPPR7 in regulating cold tolerance.
Main Methods:
- MutMap and genetic analysis to identify the causal gene.
- CRISPR/Cas9 gene editing to validate gene function.
- Cytological observation and molecular analysis of OsPPR7 function.
Main Results:
- A novel cold-sensitive mutant, csb1, was identified, linked to a promoter mutation in OsPPR7.
- OsPPR7 loss-of-function leads to defective anther development, pollen abnormalities, and sterility under cold stress.
- OsPPR7 regulates cold tolerance by modulating abscisic acid biosynthesis and reactive oxygen species homeostasis.
Conclusions:
- OsPPR7 is a critical positive regulator of reproductive stage cold tolerance in rice.
- OsPPR7's natural variation and selective sweep in temperate japonica rice suggest its role in adaptation.
- OsPPR7 represents a valuable genetic target for breeding climate-resilient rice varieties.
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