Collagen films with controlled surface topology improve corneal cell migration

Corneal damage is a significant problem in modern ophthalmology, and the repair of this damage through the transplantation of collagen films has become an attractive and promising approach in regenerative ophthalmology. We have developed a method for creating collagen films using simple and accessible techniques that enhance the migration of corneal cells. Our research has focused on investigating the effects of variables such as the concentration of chloride anions in collagen solutions and pre-cooling on the properties of these films. Using turbidity measurements, we found that pre-incubation of collagen solutions at low temperatures promotes the formation of fibrillar structures. Atomic force microscopy confirmed these findings, revealing an increase in fibrillar collagen content on the surface of the films. The proposed conditions for film formation contribute to an increase in the rate of migration of corneal cells. These films represent a promising product for use in corneal regenerative medicine.