Related Experiment Video
Updated: May 12, 2026
Retrieval of Mouse Oocytes
Published on: April 28, 2007
Transabdominal retrieval of oocytes when transvaginal access is not an option: mastering an egg-cellent technique
Ahmad Hasan1, Kelsey Zhao2, Bridget Lu2
1Division of Gynecologic Reproductive Endocrinology and Infertility, Department of Obstetrics, Medicine, University of Toronto, Toronto, Ontario, Canada; Mount Sinai Fertility, Toronto, Ontario, Canada.
Objective:
To demonstrate the step-by-step technique for performing a transabdominal oocyte retrieval with a vaginal transducer in patients where transvaginal access is not an option.
Design:
Video article.
Subjects:
The first patient is a 38-year-old gravida 0 woman with prior midline laparotomy for myomectomy and endometriosis resection. In vitro fertilization was recommended after unsuccessful natural conception attempts. Her baseline antimüllerian hormone was 17 pmol/L (2.38 ng/mL). The second patient is a 26-year-old gravida 0 woman with history of multiple abdominal and pelvic surgeries because of congenital vaginal and anal atresia. She presented with locally advanced cancer of the neovagina. Her baseline antimüllerian hormone was 24 pmol/L (3.36 ng/mL). The patients included gave consent for publication and posting of the video online including social media, the journal website, scientific literature websites (such as PubMed, ScienceDirect, and Scopus, etc.) and other applicable sites.
Exposure:
Both patients underwent controlled ovarian stimulation using a gonadotropin-releasing hormone antagonist protocol. Oocyte retrievals were performed transabdominally using a 7 MHz vaginal transducer fitted with a disposable needle guide and a 17 gauge, 25-cm single lumen aspiration needle. Follicular aspiration was performed with a pedal-activated vacuum pump with suction pressures ranging between 105-140 mmHg.
Main Outcome Measures:
Oocyte yield after transabdominal oocyte retrieval.
Results:
The first patient's in vitro fertilization cycle yielded 12 oocytes, of which 8 fertilized with conventional insemination; four blastocysts were cryopreserved. A frozen embryo transfer is planned imminently. Twenty-one oocytes were retrieved in the second patient, of which 12 mature oocytes were cryopreserved for future use. She is currently undergoing radiation therapy.
Conclusion:
Transabdominal oocyte retrieval using a vaginal transducer is a safe, effective, and feasible method of oocyte retrieval in select patients where ovaries are not accessible transvaginally. This technique may be more easily adopted where abdominal transducer needle guides and adapters are not readily accessible, offering a practical alternative for oocyte retrieval in patients with complex pelvic anatomy or pathology.
