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Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture

Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture

Published on: September 29, 2017

Multifunctional modified chitosan hydrogel with excellent comprehensive properties by one pot.

Guanghua He¹, Shilin Tian¹, Yaqian Zhou¹

¹School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.

International Journal of Biological Macromolecules

|May 10, 2026

Summary

A facile one-pot method created a multifunctional chitosan-dihydroxybenzaldehyde hydrogel with excellent adhesion, rapid in-situ gelling, and robust network structure. This antioxidant and antibacterial hydrogel demonstrates promising cytocompatibility and hemocompatibility for various applications.

Keywords:

Chitosan Comprehensive properties Hydrogel

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Area of Science:

Materials Science
Polymer Chemistry
Biomaterials Engineering

Background:

Traditional multifunctional hydrogels require complex multi-step syntheses.
A facile, aqueous-based, one-pot method is needed for efficient hydrogel preparation.

Purpose of the Study:

To develop a simple and efficient method for preparing multifunctional hydrogels.
To characterize the properties and potential applications of the synthesized hydrogel.

Main Methods:

Synthesized chitosan-dihydroxybenzaldehyde (CS-DHB) hydrogel using chitosan (CS) and 3,4-dihydroxybenzaldehyde (DHB) via a one-pot Schiff base reaction.
Utilized aluminum chloride hexahydrate (AlCl3·6H2O) as a crosslinking agent.
Characterized hydrogel structure (SEM), swelling, adhesion strength, gelation time, mechanical properties (rheology), degradation, antioxidant activity (DPPH assay), antibacterial efficacy (S. aureus, E. coli), cytocompatibility, and hemocompatibility.

Main Results:

SEM revealed interconnected pores, supporting swelling properties.
Achieved high adhesion strength (80.2 kPa) and rapid in-situ gelation (<60 s).
Demonstrated a stable network structure (G' > G'') and >80% degradation over 17 days.
Exhibited excellent antioxidant and antibacterial properties (90% against S. aureus, 98% against E. coli).
Showcased good cytocompatibility and hemocompatibility.

Conclusions:

The one-pot synthesis provides a facile and efficient route to multifunctional CS-DHB hydrogels.
The hydrogel possesses desirable properties including strong adhesion, rapid gelation, stability, degradation, potent antioxidant and antibacterial activity, and biocompatibility.
This CS-DHB hydrogel is a promising candidate for biomedical and other advanced applications.