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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Related Experiment Video

Updated: May 12, 2026

Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
09:56

Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales

Published on: August 21, 2019

Reflectance-based optical sensor for real-time detection and quantification of biofilms in complex matrices.

Sonali Rana1, Lata Sheo Bachan Upadhyay1, Nikhil Kumar2

  • 1Department of Biotechnology, National Institute of Technology Raipur, Raipur, Chhattisgarh 492010, India.

Methods (San Diego, Calif.)
|May 10, 2026
PubMed
Summary

A new portable optical sensor offers rapid bacterial biofilm detection using colorimetric analysis. This cost-efficient system provides accurate quantification for public health and environmental monitoring.

Keywords:
BiofilmLDRMethylene blueReflectance

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Last Updated: May 12, 2026

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Concurrent Quantification of Cellular and Extracellular Components of Biofilms
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Concurrent Quantification of Cellular and Extracellular Components of Biofilms

Published on: December 10, 2013

Area of Science:

  • Microbiology
  • Analytical Chemistry
  • Sensor Technology

Background:

  • Biofilm detection is crucial for public health and environmental safety.
  • Conventional methods are limited by complexity, cost, and time.
  • Reflectance-based platforms offer simpler, more accessible alternatives.

Purpose of the Study:

  • To develop a portable, cost-efficient optical sensor for bacterial biofilm detection.
  • To utilize methylene blue-mediated colorimetric analysis for quantification.
  • To provide a rapid and accurate method for on-site biofilm monitoring.

Main Methods:

  • Designed a reflectance-based optical sensor integrating a paper-based analytical device (PAD).
  • Employed a microcontroller, light-dependent resistor (LDR), LED, and OLED display for reflectance monitoring.
  • Optimized PAD with methylene blue for interaction with extracellular matrix components.

Main Results:

  • The sensor demonstrated effective interaction with bacterial extracellular matrix constituents.
  • Achieved a strong correlation (R² = 0.9946) with microtiter plate assays, indicating high accuracy.
  • Confirmed the sensor's sensitivity and reproducibility for biofilm quantification.

Conclusions:

  • The developed sensor offers a reproducible, rapid, and efficient approach for biofilm quantification.
  • This technology has potential applications in clinical diagnostics, environmental monitoring, and industrial microbiology.
  • The system provides a cost-effective solution for high-throughput biofilm screening.