Evaluating the transition from multidrug-resistant bacterial colonization to lower respiratory tract infection in
Montserrat Piñana1, M Ángeles Clari1, Gerardo Aguilar2
1Microbiology Service, Clinic University Hospital, INCLIVA Health Research Institute, Valencia, Spain.
Introduction:
We hypothesized that the use of the BioFire® FilmArray Pneumonia Plus Panel (FA-PP) may help to assess the relationship between colonization and ventilator-associated lower respiratory infection (LRTI) caused by multidrug-resistant bacteria (MDRB).
Methods:
A total of 376 patients underwent surveillance cultures for MDRB. Endotracheal aspirates were tested in parallel by the FA-PP and semiquantitative cultures for LRTI etiological diagnosis.
Results:
A total of 131 patients had MDRB recovered from surveillance cultures (34.8%), of which 10 subsequently developed LRTI caused by a matched MDRB. In 2 patients, MDRB were detected by the FA-PP (blaOXA-48-producing Klebsiella pneumoniae and ESBL-producing K. pneumoniae) that were not recovered in culture. Among non-MDRB-colonized patients with LRTI due to MDRB (n=15) the FA-PP detected MDRB in 6 patients that were later recovered in screening cultures.
Conclusion:
Incorporating FA-PP testing for LRTI diagnosis may be ancillary to assess the relationship between MDRB colonization and subsequent LRTI by matched strains.
Insights
The BioFire® FilmArray Pneumonia Plus Panel (FA-PP) can help identify links between multidrug-resistant bacteria (MDRB) colonization and ventilator-associated lower respiratory infection (LRTI). This diagnostic tool aids in understanding MDRB transmission in critical care settings.
Area of Science:
- Infectious Diseases
- Microbiology
- Clinical Diagnostics
Background:
- Ventilator-associated lower respiratory infection (LRTI) is a significant concern in critical care.
- Multidrug-resistant bacteria (MDRB) pose a growing threat, complicating LRTI diagnosis and treatment.
- Understanding the relationship between MDRB colonization and subsequent LRTI is crucial for effective infection control.
Purpose of the Study:
- To evaluate the utility of the BioFire® FilmArray Pneumonia Plus Panel (FA-PP) in assessing the link between MDRB colonization and LRTI.
- To compare the diagnostic performance of FA-PP with traditional culture methods for MDRB detection in LRTI.
Main Methods:
- A prospective study involving 376 patients requiring surveillance for MDRB.
- Endotracheal aspirates were analyzed in parallel using FA-PP and semiquantitative cultures.
- Analysis focused on identifying MDRB colonization and subsequent LRTI caused by matched strains.
Main Results:
- MDRB colonization was detected in 34.8% of patients (131/376).
- Ten patients developed LRTI caused by MDRB strains matching surveillance cultures.
- FA-PP identified MDRB in 2 patients missed by culture and detected MDRB in 6/15 non-colonized patients who later developed MDRB LRTI.
Conclusions:
- The FA-PP shows promise as an ancillary tool for diagnosing LRTI.
- It can aid in assessing the relationship between MDRB colonization and the development of LRTI by matched strains.
- Integrating FA-PP into LRTI diagnostic workflows may improve the understanding and management of MDRB infections.
More Related Videos
07:16Development of a Polymicrobial Colony Biofilm Model to Test Antimicrobials in Cystic Fibrosis
Published on: September 20, 2024
09:26Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung
Published on: January 22, 2021
