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Study of Oxytetracycline Using Chitosan-Modified Magnetic Molecularly Imprinted Polymers Combined With
Hui Yuan1, Tai Feng Lin1, Xue Mei Liu2
1College of Chemistry and Life Science, Beijing University of Technology, Beijing, China.
Abstract:
Human consumption of food contaminated with oxytetracycline (OTC) residues can lead to its accumulation in the body and cause harm. In this study, due to the trace residues of OTC in food, surface imprinting technology was employed to prepare oxytetracycline magnetic molecularly imprinted polymers (OTC-MMIPs). This was achieved using Fe3O4@SiO2 as the core, modified with chitosan, and with OTC as the template molecule. OTC-MMIPs were used to adsorb OTC. After combining with AuNPs, the OTC-MMIPs@AuNPs composite was used to adsorb OTC, and then SERS was employed for detection. According to the kinetic adsorption experiment, the maximum adsorption capacity of OTC-MMIPs was 44.58 mg g-1, with a 7% decrease in adsorption capacity observed after four cycles of reuse. Following the adsorption of OTC onto OTC-MMIPs@AuNPs, detection was performed using SERS. A linear relationship was established between the intensity of the characteristic peak at 1329 cm- 1 and the OTC concentration, achieving a detection limit of 6.67 × 10-12 M. Furthermore, the method was validated in pure milk samples, the recovery rate ranges from 91.73% to 96.22%. These results demonstrate the feasibility of the proposed method for detecting OTC in pure milk.
Insights
This study developed magnetic molecularly imprinted polymers (OTC-MMIPs) for detecting oxytetracycline (OTC) residues in food. The OTC-MMIPs@AuNPs composite achieved highly sensitive detection of OTC, showing promise for food safety analysis.
Area of Science:
- Analytical Chemistry
- Materials Science
- Food Science
Background:
- Oxytetracycline (OTC) residues in food pose health risks due to potential bioaccumulation.
- Sensitive detection methods are crucial for monitoring OTC contamination in food products.
Purpose of the Study:
- To develop a novel method for sensitive detection of trace oxytetracycline (OTC) residues in food.
- To create and characterize oxytetracycline magnetic molecularly imprinted polymers (OTC-MMIPs) for enhanced adsorption and detection.
Main Methods:
- Surface imprinting technology was used to synthesize OTC-MMIPs using Fe3O4@SiO2 core and chitosan modification.
- OTC-MMIPs were combined with gold nanoparticles (AuNPs) to form OTC-MMIPs@AuNPs composite.
- Surface-enhanced Raman spectroscopy (SERS) was employed for sensitive OTC detection.
Main Results:
- The OTC-MMIPs exhibited a maximum adsorption capacity of 44.58 mg g⁻¹ with good reusability (7% capacity decrease after four cycles).
- The OTC-MMIPs@AuNPs composite coupled with SERS achieved a low detection limit of 6.67 × 10⁻¹² M for OTC.
- The method demonstrated high recovery rates (91.73%–96.22%) in pure milk samples.
Conclusions:
- The developed OTC-MMIPs@AuNPs composite and SERS method offer a feasible and highly sensitive approach for detecting OTC residues in food matrices.
- This technology holds significant potential for ensuring food safety and protecting public health from antibiotic contamination.
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