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Antimicrobial photodynamic inactivation (API) using LED light and a photosensitizer reduced biocide-resistant sulfate-reducing bacteria (SRB) in oilfields. This shows API

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Area of Science:

  • Microbiology
  • Environmental Science
  • Biotechnology

Background:

  • Sulfate-reducing bacteria (SRB) cause biogenic acidification, degrading oil quality and posing economic/environmental challenges.
  • Biocide resistance in SRB strains necessitates alternative control strategies to prevent environmental contamination.

Purpose of the Study:

  • To evaluate antimicrobial photodynamic inactivation (API) as a control method for biocide-resistant SRB.
  • To assess API efficacy under conditions mimicking oilfield microbial communities.

Main Methods:

  • Utilized 1,9-Dimethyl-methylene blue (DMMB) as a photosensitizer with LED light activation (630 nm).
  • Tested DMMB concentrations (1.0-2.0 μg/mL) and energy densities (8.0-21.6 J/cm²).
  • Investigated API mechanism (Type I) and factors affecting efficacy, including bacterial interactions and H₂S quenching.

Main Results:

  • API treatment reduced SRB log counts by up to 72.86% (0.57 log) within 9 minutes.
  • Antimicrobial effects were attributed to reactive oxygen species (ROS) and hydroxyl radical generation.
  • Incomplete inhibition was linked to DMMB-lipopolysaccharide interactions and H₂S quenching of singlet oxygen.

Conclusions:

  • API demonstrates potential for controlling biocide-resistant SRB in oilfield environments.
  • Optimizing photosensitizer choice and photoactivation conditions is crucial for API effectiveness.
  • Understanding interactions within complex microbial consortia, including quenchers like H₂S, is key for successful API application.