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Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
Molecular characterization, function, tissue differential expression, and single-nucleotide polymorphism of buffalo
Lige Huang1, Dan Sheng1, Xinyang Fan1
1Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China.
Archives Animal Breeding
|May 11, 2026
Summary
The TP53 gene plays a crucial role in buffalo lactation, negatively regulating milk protein and fat synthesis. This study characterized the buffalo TP53 gene, revealing its function and expression patterns during lactation.
Area of Science:
- Genomics and Molecular Biology
- Animal Science
- Biochemistry
Background:
- The role of the TP53 gene in cattle lactation is known, but its function in buffaloes remains unexplored.
- Understanding TP53's involvement in buffalo lactation can provide insights into milk production regulation in this species.
Purpose of the Study:
- To isolate and characterize the complete coding sequence (CDS) of the TP53 gene in buffalo mammary glands.
- To analyze the molecular characteristics, function, tissue expression, and genetic variations of buffalo TP53.
- To elucidate the role of TP53 in regulating lactation in buffaloes.
Main Methods:
- Isolation and cloning of the buffalo TP53 gene's complete coding sequence (CDS).
- Bioinformatics analysis for molecular characteristics, conserved domains, and protein structure.
- Quantitative analysis of TP53 gene expression across 11 buffalo tissues during lactation and non-lactation.
- Functional experiments using buffalo mammary epithelial cells (BuMECs) to investigate TP53's regulatory pathways (PI3K-AKT-mTOR).
- Identification and analysis of single-nucleotide polymorphisms (SNPs) in the TP53 CDS.
Main Results:
- The buffalo TP53 gene CDS is 1161 bp, encoding a 386-amino acid protein with high similarity to other Bovidae species.
- TP53 is expressed in all detected buffalo tissues, with significantly higher expression in heart, kidney, brain, muscle, and rumen during lactation.
- TP53 expression was lower in the liver, lung, and mammary gland during lactation compared to non-lactation.
- Interference experiments demonstrated that TP53 inhibits milk protein and fat synthesis genes via the PI3K-AKT-mTOR pathway.
- A synonymous SNP (c.204C>T) was identified in river buffalo TP53 CDS, corresponding to the swamp buffalo CC genotype.
Conclusions:
- The TP53 gene is significantly involved in buffalo lactation.
- TP53 negatively regulates milk protein and milk fat synthesis pathways in buffaloes.
- The characterized buffalo TP53 gene and its identified SNP provide a basis for further research into lactation regulation and breeding strategies.
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