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Dynamic full-field swept-source optical coherence tomography for high-resolution, long-depth, and
Nobuhisa Tateno1, Yue Zhu1, Suzuyo Komeda1
1Computational Optics Group, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Biomedical Optics Express
|May 11, 2026
Summary
A new dynamic spatially-coherent full-field OCT (SC-FFOCT) overcomes depth limitations and visualizes intra-tissue activities. This advanced optical coherence tomography technique achieves high-resolution 3D imaging in biological samples.
Area of Science:
- Biomedical Optics
- Optical Imaging
- Cell Biology
Background:
- Optical coherence tomography (OCT) offers high resolution but is limited by depth of focus and signal reduction.
- Standard OCT cannot visualize dynamic intra-tissue processes.
- Existing methods struggle with imaging depth and real-time cellular activity.
Purpose of the Study:
- To develop a novel OCT modality overcoming depth and dynamic imaging limitations.
- To visualize three-dimensional structure and intra-tissue activities simultaneously.
- To demonstrate the capability of the new technique in biological samples.
Main Methods:
- Development of dynamic spatially-coherent full-field OCT (SC-FFOCT).
- Implementation of computational refocusing to extend imaging depth.
- Utilization of a repetitive acquisition protocol for dynamic OCT imaging.
Main Results:
- Achieved in-focus lateral resolution of 1.4 µm and axial resolution of 6.5 µm.
- Demonstrated extended imaging depth through computational refocusing.
- Successfully visualized dynamic intra-tissue activities in human breast adenocarcinoma spheroids (MCF-7 cell line).
Conclusions:
- Dynamic SC-FFOCT effectively overcomes the depth limitations of conventional OCT.
- The modality enables high-resolution, volumetric, dynamic imaging of biological structures.
- SC-FFOCT provides a powerful tool for studying cellular dynamics in 3D within biological tissues.
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