Modulated Doppler phase microscopy for dynamic imaging with subcellular resolution

Area of Science:

• Biomedical Optics
• Nanotechnology
• Cellular Imaging

Background:

• Cell-nanoparticle interactions are vital for nanoparticle-based medicine.
• Doppler Optical Coherence Tomography (DOCT) is used for dynamic imaging but has resolution limitations for en face motion.
• Conventional DOCT requires oversampling and struggles with high-resolution, high-sensitivity motion imaging.

Purpose of the Study:

• To develop a novel imaging technology for high-resolution, high-sensitivity motion imaging of cell-nanoparticle interactions.
• To overcome the spatial and temporal resolution limitations of conventional DOCT for en face plane imaging.
• To enable precise detection of nanoparticle motion at the cellular level.

Main Methods:

• Developed modulated Doppler phase microscopy (M-DPM) technology.
• Employed an optical computation strategy involving Fourier transform of the interferometric spectrum.
• Applied temporal modulation and time-domain filtering to 3D data (x-y-t) for phase-resolved Doppler imaging.
• Utilized M-DPM for en face plane imaging.

Main Results:

• Achieved high-resolution and high-sensitivity motion imaging in the en face plane.
• Validated M-DPM performance using samples with global motion.
• Demonstrated capability for spatially resolved motion detection by imaging magnetic particles interacting with cells.

Conclusions:

• M-DPM is an effective technology for high-resolution, high-sensitivity Doppler imaging in the en face plane.
• The developed M-DPM overcomes limitations of conventional DOCT for cellular motion analysis.
• M-DPM shows promise for studying dynamic cell-nanoparticle interactions in biomedical applications.