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Related Experiment Video

Updated: May 12, 2026

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
10:59

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Published on: May 28, 2021

Lipid Analysis in Live Caenorhabditis elegans Using Solution-State NMR Spectroscopy.

Florencia V Guastaferri1, Carla B Delprato2, Bruno Hernández Cravero2

  • 1Cellular-Structural Biology Lab., Institute of Molecular and Cellular Biology of Rosario (IBR-CONICET-UNR), Ocampo y Esmeralda, Rosario, Argentina.

Bio-Protocol
|May 11, 2026
PubMed
Summary

This study introduces a non-destructive nuclear magnetic resonance (NMR) method for analyzing lipid composition and unsaturated fatty acids (UFAs) in live Caenorhabditis elegans. The technique allows for in vivo assessment of lipid metabolism and distinguishes between wild-type and altered fatty acid desaturation.

Keywords:
13C isotope labelingCaenorhabditis elegansIn vivo NMR spectroscopyLipidsUnsaturated fatty acids (UFAs)

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Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans
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Last Updated: May 12, 2026

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10:59

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Published on: May 28, 2021

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Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans
16:07

Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

Published on: March 30, 2013

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Metabolomics

Background:

  • Unsaturated fatty acids (UFAs) are crucial for cellular functions, and their metabolic disruptions are linked to various diseases.
  • Current methods for UFA analysis, like gas chromatography and mass spectrometry, require sample extraction and cannot analyze live organisms.
  • Investigating lipid metabolism in vivo is essential for understanding cellular health and disease.

Purpose of the Study:

  • To develop a non-destructive method for analyzing lipid composition and fatty acid unsaturation directly in live Caenorhabditis elegans.
  • To enable in vivo assessment of lipid metabolism with high resolution and sensitivity.
  • To provide a tool for studying lipid metabolism in small model organisms.

Main Methods:

  • Utilizing uniform 13C isotope labeling and high-resolution 2D solution-state nuclear magnetic resonance (NMR) spectroscopy.
  • Analyzing lipid composition and fatty acid unsaturation directly in live Caenorhabditis elegans.
  • Complementary analysis of total lipid extracts for comprehensive lipid profiling.

Main Results:

  • The NMR-based approach allows for in vivo assessment of lipid storage compositions in live C. elegans.
  • The method possesses sufficient resolution and sensitivity to differentiate wild-type animals from those with altered fatty acid desaturation.
  • NMR analysis of lipid extracts provides information on lipid molecules not detectable in vivo, such as membrane phospholipids.

Conclusions:

  • This non-destructive NMR-based method is a powerful tool for investigating lipid metabolism in C. elegans and other small, isotopically enrichable model systems.
  • The technique enables sensitive and precise interpretation of whole-cell lipid metabolism in live animals.
  • Solution-state NMR can be applied to live cells and multicellular organisms, offering new avenues for biological research.