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Optimizing Component Formula Extracted from Ginseng and Salvia Inhibits Lung Cancer Metastasis and EMT via
Yanxia Ma1, Ze Chen1, Tangxiaoxiao He1
1School of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
Background:
EMT plays a pivotal role in the metastatic progression of lung cancer. Ginseng and salvia are widely used combinations in Chinese medicine for cancer therapy, but their role in regulating cancer metastasis remains unknown.
Purpose:
This investigation seeks to examine the efficacy and potential mechanism of optimizing component formula (OCF) extracted from ginseng and salvia on metastasis-associated EMT reprogramming in lung cancer.
Methods:
The inhibitory effects of OCF on tumor growth and metastatic cascades were systematically characterized in vitro. The tail vein injection method for lung metastasis model was used to observe anti-tumor metastasis effect of OCF in vivo. Molecular profiling involving PCRArray screening, quantitative PCR verification, and immunoblotting analysis elucidated the mechanistic basis.
Results:
OCF markedly suppressed the proliferative capacity, migratory and invasive behaviors in H1299 and 95D cells. Signal Transduction PathwayFinder PCRArray and qRT-PCR analysises confirmed that suppressing EMT was a potential way of OCF to inhibit metastasis in lung cancer. Mechanistically, OCF exhibited a good affinity with AKT resulting in inhibition of the AKT/GSK-3β/β-catenin pathway. Co-treatment of OCF with SC79 or MK-2206 demonstrated that OCF weakened EMT progress via repressing AKT/GSK3β/β-catenin pathway in lung cancer. Furthermore, OCF treatment inhibited lung cancer growth and metastasis in vivo. Importantly, IHC and IF assays showed that OCF has also restrained the development of EMT and AKT/GSK3β/β-catenin pathway.
Conclusion:
OCF weakened EMT progression in lung cancer by suppressing AKT/GSK-3β/β-catenin pathway, which positions OCF as a therapeutic candidate for lung cancer.
Insights
Optimizing Component Formula (OCF) from ginseng and salvia inhibits lung cancer metastasis by suppressing epithelial-mesenchymal transition (EMT). OCF targets the AKT/GSK-3β/β-catenin pathway, showing therapeutic potential for lung cancer treatment.
Area of Science:
- Oncology
- Pharmacology
- Molecular Biology
Background:
- Epithelial-mesenchymal transition (EMT) is crucial for lung cancer metastasis.
- Ginseng and salvia are traditional Chinese medicines with unknown effects on cancer metastasis.
Purpose of the Study:
- To investigate the efficacy of Optimizing Component Formula (OCF) on metastasis-associated EMT in lung cancer.
- To elucidate the mechanism by which OCF affects lung cancer progression.
Main Methods:
- In vitro characterization of OCF effects on cell proliferation, migration, and invasion.
- In vivo studies using a tail vein injection lung metastasis model.
- Molecular profiling including PCRArray, qRT-PCR, and immunoblotting.
Main Results:
- OCF significantly suppressed lung cancer cell proliferation, migration, and invasion.
- OCF inhibited EMT and lung cancer metastasis by targeting the AKT/GSK-3β/β-catenin pathway.
- OCF demonstrated anti-tumor and anti-metastasis effects in vivo.
Conclusions:
- OCF weakens EMT progression in lung cancer by suppressing the AKT/GSK-3β/β-catenin pathway.
- OCF is a potential therapeutic candidate for treating lung cancer.