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Updated: May 12, 2026

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
Published on: December 20, 2017
Identification of a potential internalization or endocytic compartment-targeting signal in the C-terminal tail of the
Zhiyu Liu1, Byron Williams1, Jun Liu1
1Department of Molecular Biology and Genetics, Cornell University.
Abstract:
Tetraspanins are unique yet highly conserved four-pass transmembrane proteins that play important roles in diverse biological processes. The C. elegans tetraspanin protein TSP-12 is localized both at the cell surface and in multiple intracellular compartments, including different endosomal vesicles and the Golgi. In this study, we found that mutating key residues in the short C-terminal intracellular domain of TSP-12 (ILxxxxxWYY) led to an increase of cell surface localization of TSP-12 without affecting its localization in the ER or Golgi. These observations suggest that the ILxxxxxWYY motif in TSP-12 represents a potential internalization or endocytic compartment-targeting signal.
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