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Related Experiment Video

Updated: May 12, 2026

Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems
09:28

Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems

Published on: November 4, 2014

A comprehensive method for quantifying human cytomegalovirus plaque assays.

Laurel E Kelnhofer-Millevolte1,2, Daniel H Nguyen3,4, Lea S Wilson3,4

  • 1Molecular and Cellular Biology, Graduate Program, University of Washington and Fred Hutchinson Cancer Center, Seattle, WA, United States.

Frontiers in Cellular and Infection Microbiology
|May 11, 2026
PubMed
Summary

This study introduces a new ImageJ-based pipeline for quantifying human cytomegalovirus (hCMV) plaques. This method offers a standardized and objective approach to measure infectious viral progeny, aiding hCMV research.

Keywords:
herpesvirushuman cytomegalovirus (CMV)imaginginfectionplaque assay

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Related Experiment Videos

Last Updated: May 12, 2026

Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems
09:28

Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems

Published on: November 4, 2014

Plaquing of Herpes Simplex Viruses
04:41

Plaquing of Herpes Simplex Viruses

Published on: November 5, 2021

Optimization of a Quantitative Micro-neutralization Assay
10:09

Optimization of a Quantitative Micro-neutralization Assay

Published on: December 14, 2016

Area of Science:

  • Virology
  • Cell Biology
  • Infectious Diseases

Background:

  • Human cytomegalovirus (hCMV) causes significant illness in immunocompromised individuals.
  • hCMV manipulates host gene expression and produces viral proteins to establish infection.
  • The plaque assay is the standard method for quantifying infectious hCMV progeny.

Purpose of the Study:

  • To develop a broadly applicable pipeline for quantifying hCMV plaques.
  • To leverage the fluorescent properties of crystal violet for plaque imaging.
  • To provide a standardized, objective method for measuring infectious viral progeny.

Main Methods:

  • Utilized crystal violet staining and fluorescent imaging of hCMV plaques.
  • Developed an ImageJ-based software pipeline for plaque quantification.
  • Applied the pipeline to analyze plaque number and size.

Main Results:

  • Successfully imaged hCMV plaques using fluorescent modalities.
  • Developed and validated an ImageJ pipeline for objective plaque quantification.
  • Demonstrated the pipeline's utility in standardizing the measurement of infectious progeny.

Conclusions:

  • The developed ImageJ pipeline offers a standardized and objective method for quantifying hCMV plaques.
  • This approach enhances the understanding of hCMV infectious progeny production.
  • Facilitates more accurate research into hCMV pathogenesis and antiviral strategies.